XI - Edition 2007

XI School of Pure and Applied Biophysics

on

Advanced Optical Microscopy Methods in Biophysics

 


Venice, - Campo Santo Stefano, January 29 - February 2, 2007

Organised by:

SIBPA- Società Italiana di Biofisica Pura e Applicata
IVSLA - Istituto Veneto di Scienze Lettere ed Arti

Scientific Coordinators

Director of the School

 School overview and definitive program


Biophysics is a molecular science rapidly moving to the nanoscale. It seeks to explain biological function in terms of the molecular structures and properties of specific molecules. The size of these molecules varies dramatically, from small fatty acids and sugars (~1 nm = 10-9 m), to macromolecules like proteins (5-10 nm), starches (bigger than 1000 nm), and the enormously elongated DNA molecules. Much effort in biophysics is directed to determining the structure of specific biological molecules and of the larger structures into which they assemble. Some of this effort involves inventing new methods and building new instruments for monitoring these structures, and many of the exciting new developments in optical microscopy, in terms of imaging and manipulation, spectroscopy and visualization, are part of this effort. Starting from these considerations we planned to focus on Advanced Optical Microscopy Methods in Biophysics the theme of the XI School of Pure and Applied Biophysics, to be held in Venice, from January 29 to February 2 - 2007, Palazzo Franchetti, the prestigious premises of the Istituto di Scienze Lettere e Arti (IVSLA).

The School is co-organized by Alberto Diaspro (Department of Physics, University of Genoa) and Cesare Usai (Institute of Biophysics, National Research Council).

Topics of the School will be on:
CARS (Coherent Anti Raman Scattering), TIRF (Total Internal Reflection Fluorescence), SHG-THG (Second-Third Harmonic Generation), Correlative microscopy, Multiphoton microscopy, Confocal Microscopy, 3D microscopy, Lifetime Imaging Microscopy, FRET (Forster resonance Enrgy Transfer), FRAP (Fluorescence Recovery After Photobleaching), SIngle molecule imaging, Tissue imaging, Cell imaging, Inverse problems in Optical Microscopy (Computational methods in image recovery), 7D Microscopy (adding new dimensions to x-y-z-t), Nanoscopy, Optical Tweezers, Complementary methods, Fluorescence Spectroscopy.

Provisional list of Lecturers includes:

MAIN SPONSOR: Leica Microsystems, Germany.
SUPPORTING SPONSORS (to be updated): ISS, Urbana, Illinois; Becker and Hickl; OKO-LAB ; Coherent; Springer and Verlag; Chroma; Amici del Festival della Scienza; Comune di Genova.



REGISTRATION
The number of students admitted is restricted to 30.
The participation fee is 300 Euros which includes five nights accommodation and attendance at the lessons. The participation fee is reduced to 150 Euros in case the attendant does not need accommodation. The participation fee is reduced to 250 Euros for those registering by 15 December 2006.
Applicants are admitted to the School by the Scientific Committee based on the information (short curriculum) provided on the pre-registration form, which must be submitted on line using the following application form.
The admittance will be individually communicated via e-mail.

FURTHER DETAILS AND UPDATES
http://www.lambs.it (section Events)

Timetable

Timetable

Mon – Jan 29

Tue - Jan30

Wed – Jan 31

Thu – Feb 1

Fri – Feb 2

8.45 -9.45

 

Basics of Fluorescence Spectroscop

R. Bizzarri

Laser Sources in Microscopy. Advances.

C. Dorman

Fluorescence lifetime imaging

W. Becker

Advanced biomedical applications of carbon nanotubes.

V. Mussi

9.45-10.45

Arrival

and

Registration

Fundamentals of Optical Microscopy

F.Quercioli

AO and fiber laser - ingenious inventions in microscopy

R.Borlinghaus

Title to be announced

T. Pozzan

Fluorescence Nanoscopy: 4PI and STED

S. Hell

10.45-11.00

WELCOME

Coffè time

Coffè time

Coffè time

Coffè time

11.00-12.00

Recent Advances in Single Molecule Manipulation Biophysics

C. Bustamante

Confocal and Two-photon excitation Notes

P. Bianchini

FRET imaging Notes

V.Caorsi

Linear and Non-linear activation of PA- GFP

I.Testa

Fluorescence Nanoscopy

RESOLFT - unlimited resolution.

S. Hell

12.00-13.00

Nanoscience to learn biology, biology to develop nanotechnology.

 

B. Samorì

Fast random-access scanning for 1-/M-photon

microscopy

Peter Saggau

Analysis of FRET signals.

E.Neher

Fluorescence Recovery After Photobleaching

D.Mazza

FAREWELL

(15')

13.00-14.30

Lunch time

Lunch time

Lunch time

Lunch time

Lunch time

14.30-15.30

Early confocal/sectioning microscopy

G.J. Brakenhoff

3D imaging with high spatial resolution..

M. Martinez-Corral

Imaging in 3D Fluorescence Microscopy:

G.Vicidomini

A window with a view: 2PE in thick tissues

G.Ratto

 

15.30-16.30

Making Light work in Microscopy

T. Wilson

Total Internal Reflection Fluorescence Microscopy

D. Parazzoli

Sectioning microscopy with thin uniform fluorescent layers: SIPcharts

G.J. Brakenhoff

Advanced uses of fluorescent proteins in the investigation of cells

F.Wouters

-

16.30-17.00

Tea time

Tea time

Tea time

Tea time

-

17.00-18.00

In vivo and in vitro Optical Manipulation

F. Pavone

SHG and CARS Microscopy I

F. Pavone

3-D analysis of biostructures by stereology and other methods.,

L.Kubinova

5D. towards imaging of complexity

M. Faretta

-

18.00-19.00

Single Molecule Imaging

G. Chirico

SHG and CARS Microscopy II

F.Pavone

Confocal Microscopy of Ciliated Protozoa

G.Colombetti

QDs as Probes for Biomolecular Targets

B. Knight

 


Further information can be obtained from:
 
Alberto Diaspro
Dept. of Physics
University of Genoa
Via Dodecaneso, 33
16146 Genova - ITALY
Phone:  +39 010 3536426 
Fax: +39 010 2099227
E-mail: diaspro(at)fisica.unige.it

Cesare Usai
CNR - Institute of Biophysics, Unit at Genoa
Via De Marini, 6
16146 Genova - ITALY
Phone:  +39 010 6475579 
Fax: +39 010 6475500
E-mail: usai(at)nameserver.ge.cnr.it

and from the director of the school:

Giorgio M. Giacometti
Dept. of Biology
University of Padova
Viale G. Colombo, 3
35131 Padova - ITALY
Phone:  +39 049 8276326 
Fax: +39 049 8276300
E-mail: gcometti(at)bio.unipd.it